하악두의 부분절제된 점골 및 골의 치료과정에서 비료원성 단백질 분포에 관한 연구-주사전자현증경적 및 면역조직화학적 연구-
Distribution of noncollagenous protein during repair of partially resected condylar cartilage and bone-Sem and immunohistochemical study-
곽춘길, 양익,
소속 상세정보
곽춘길 ( ) - 경희대학교
양익 ( ) - 경희대학교
KMID : 0360119960180030411
Abstract
The purpose of this study was to observe the healing process and the distribution of fibronectin in injured condylar cartilage and bone by using LM and SEM. In order to perform this study, 40 male rat, weighing about 250g were selected. Under
general
anesthesia with Pentobarbital sodium, condylar cartilage and neck bone were resected. Then, the wound was irrigated with saline and closed with 5-0 chromic catgut and 4-0 silk by layer-to-layer suturing.
The experimental rats were sacrificed by perfusion with 3% paraformaldehyde at 1st and 4th week after operation.
The condylar process and surrounding tissues were cut, demineralized, dehydrated and embedded in paraffin. The histological observation of the specimens in LM level was performed after H-E stain and Azan stain. For localization of fibronectin,
immunostaining was achieved by the avidin-biotin complex method. To study the change on condylar surface, the specimens were dehydrated, dried, gold coated and were observed with a scanning electron microscope(Hitachi S-2300).
@ES The results were as follows ;
@EN 1. The cartilage group and the bone group were repaired with epiphyseal cartilage layer on the cut surface as the normal control group.
2. The cut surface was repaired more quickly in the cartilage group than in the bone group.
3. Chondrocytes, diferentiated during healing, were stained strongly to anti-fibronectin, and fibronectin was supposed to participatein chondrocyte differentiation and cartilagenous matrix formation.
4. Fibronectin was distributed more in the new bone than in the old bone, and the osteoblasts surrounding it were also stained strongly. Fibronectin was supposed to participate in new bone matrix formation.
5. Fibronectin is supposed to be associated with the differentiation, migration and adhesion of chondrocyte and osteoblast and to participate in endochondral bone formation.
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